Serial dilution calculator
From your starting concentration to the target: steps, vials and volumes to pipette.
Parameters
Starting concentration (stock)
Target concentration
Final volume needed
ml
µl
Strategy
Factor per step
Number of steps
Default dilution per step
Number of steps
(equal factor)
Adjustment step
Last step
First step
Advanced options
Diluent
Minimum pipetting volume
(µl)
Vial capacity
(ml)
Residual volume left in intermediate vials
(µl)
Excess on final volume
(%)
Examples:
Endotoxin 1000 → 0.5 EU/ml
Protein 1 mg/ml → 50 ng/ml
Drug 10 mM → 25 nM